| BMG;β2-MG

β2 microglobulin (BMG; β2-MG) is encoded by the B2M gene. Beta2 microglobulins bind not only to the α chain of MHC Class I molecules, but also to Class I molecules such as CD1, MR1, and Qa-1 (a form of alloantigen). Another function of it is that it is associated with the HFE protein and co-regulates the expression of hemoglobinin in the liver, which degrades against the iron transporter ferroportin on the basement membrane of intestinal cells and macrophage cell membranes, resulting in reduced iron uptake from food and iron release from red blood cells recycled from MPS (mononuclear phagocytic system), respectively. Loss of this function can lead to iron excess and hemochromatosis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.1	1.46	0.95	0.61	0.4	0.28	0.22	0.08
Corrected OD	2.02	1.38	0.87	0.53	0.32	0.2	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse BMG;β2-MG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse BMG;β2-MG and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108